Fig. 4. Staining for osteogenic, adipogenic and chondrogenic differentiation of control and inflammatory-stimulated eBM-MSCs from three experiments. (A) Alizarin red staining of eBM-MSCs cultured for 7 days in osteogenic differentiation medium from control and Experiment 1–3. (B) Oil red O staining of eBM-MSCs cultured for 15 days in adipogenic differentiation medium from control and Experiment 1–3. (C) Haematoxylin and Alcian blue staining of pellets cultured for 21 days in chondrogenic medium from control and Experiment 1–3. Scale bars = 500 µm (A and B), 2 µm (C). 10× (A), 4× (B), 20×(C).