Table 1.
Bacterial strains, cell lines, plasmids, and oligos used in this study.
| Bacterial strains | Description | Source/Reference |
|---|---|---|
| Lactobacillus reuteri | Wild type (GTFW) | American Type |
| ATCC 23272 | Culture Collection | |
| LMW500 | L. reuteri 23272 ΔgtfW; CmR | This study |
| LMW501 | L. reuteri 23272 + pWAR501 CmR | This study |
| LMW502 | E. coli ER2566 + pWAR502 | This study |
| LMW503 | L. reuteri 23272 + pWAR503 CmR | This study |
| Lactobacillus rhamnosus | Wild type (non-dextran forming GTF) | G. Rajashekara |
| GG ATCC 53102 | J.S. Gunn | |
| Salmonella enterica | Wild type (non-dextran forming GTF) | |
| serovar typhi TY2 ATCC | ||
| 700931 | ||
| Citrobacter rodentium | Wild type (non-dextran forming GTF) | American Type |
| ATCC 51459 | Culture Collection | |
| Clostridium difficile | Wild type (non-dextran forming GTF) | J.K. Spinier |
| R20291 (BI/NAP1/027) | ||
| Human cell lines | Description | Source/Reference |
| DLD-1 ATCC CCL-221 | Human colonic epithelial cells (colorectal adenocarcinoma) | G.E. Besner |
| FHs 74 Int ATCC CCL-241 | Human fetal small intestinal epithelial cells | G.E. Besner |
| Plasmids | Description | Source/Reference |
| pWAR500 | pFED760 (Mashburn-Warren et al., 2012) derivative containing cat and DNA fragments flanking gtfW to create insertion mutant; see Materials and Methods; CmR, ErmR | This study |
| pWAR501 | pJC156 (Mashburn-Warren et al., 2012) derivative containing the promoter region of gtfW upstream of the click beetle luciferase; see Materials and Methods; CmR | This study |
| pWAR502 | pTXB1 derivative containing gtfW (with its stop codon); see Materials and Methods; AmpR | |
| pWAR503 | pJC156 (Mashburn-Warren et al., 2012) derivative containing the promoter region of elongation factor Tu (EF-Tu) upstream of the click beetle luciferase; see Materials and Methods; CmR | This study |
| Oligos | DNA sequence* | Source/Reference |
| oSG1082 | GCGTGGCGGCCGCCATTATTTTCATGTAGTGTATT T | This study |
| oSG1083 | GCGTGGTCGACCTTTTTTATGTCCATAATCTATT | This study |
| oSG1084 | GCGTGGTCGACGAAAATATTTAATATGAAAATGA | This study |
| oSG1085 | GCGTGCTCGAGCCAAGCACTATTTCACGAGAAT | This study |
| LMW34 | GCGTGGTCGACGATGAAAATTTGTTTGATTT | Mashburn-Warren et al., 2012 |
| LMW35 | GCGTGGTCGACTTATAAAAGCCAGTCATTAG | Mashburn-Warren et al., 2012 |
| oSG1102 | GCGTGCTCGAGCAACAAGAGTATCAGGGTAAAGC | This study |
| oSG1103 | GCGTGGTCGACTCCTTCCCAATAGATGATTGATT | This study |
| oSG1067 | GCGTGGTCGACATGGTAAAACGTGAAAAAAATGT | This study |
| oSG1068 | GCGGCCGCTCCGCCAGCTTTTTCTAATAACT | This study |
| oSG1120 | GCGTGGCTAGCATGAACCTGCCAACAATTCCTAA | This study |
| oSG1126 | GCGTGGCTCTTCCGCATTAAATATTTTCTTGGTTT | This study |
| oSG1069 | GCGTGCTCGAGCGCAACAAATACAGTTTCTAATA | This study |
| oSG1070 | GCGTGGTCGACAAACCTCCTGATAATTTACAAGT | This study |
CmR, chloramphenicol resistant; ErmR, Erythromycin resistant; AmpR, Ampicillin resistant.
*
Sequences in bold indicate restriction enzyme sequences.