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. 2017 Mar 27;8:316. doi: 10.3389/fimmu.2017.00316

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Copyright © 2017 Barbarin, Cayssials, Jacomet, Nunez, Basbous, Lefèvre, Abdallah, Piccirilli, Morin, Lavoue, Catros, Piaggio, Herbelin and Gombert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD16 and CD161 are slightly enriched in innate CD8(+) T cells. (A) Peripheral blood mononuclear cells (PBMCs) from healthy donors (HDs) (n = 6) were cultured for 48 h in the presence of IL-15 and then analyzed by flow cytometry. One representative sample is shown for CD16 expression among total (upper panel) and innate (lower panel) CD8(+) T-cell subsets. Frequencies are displayed in the gate and the histogram (right panel) presents the full cohort of CD16-positive cells (mean ± SD) in both T-cell subsets. (B) PBMCs from HD (n = 9) were analyzed ex vivo by flow cytometry. One representative sample is shown for CD161 expression among total (upper panel) and innate (lower panel) CD8(+) T-cell subsets. Frequencies are displayed in the gates (left panel). The histogram (right panel) represents the full cohort of CD161-positive cells (mean ± SD) in both T-cell subsets.