Figure 2.

Peroxisome proliferator-activated receptor delta (PPARδ), 5′ adenosine monophosphate-activated protein kinase (AMPK), 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSDH1), tumor necrosis factor-alpha (TNF-α), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), medium chain acyl-CoA dehydrogenase (MCAD), and malonyl-CoA decarboxylase (MCD) protein expression in HepG2 cells treated with high fatty acid and fimasartan. Protein extracts were electrophoresed in 10% polyacrylamide gel and blotted to nitrocellulose membrane. The nitrocellulose membrane was bound with primary and secondary antibodies sequentially, and then the chemiluminescent signal was exposed to X-ray film. The density of bands on X-ray film was analyzed with ImageJ program. The results are expressed as means ± SEM (n = 3). Values were statistically analyzed by unpaired t-test. All experiments were repeated three times.