Figure 6.

The number of adipocytes and adiponectin concentration in visceral fat tissues of WKY rats and SHRs given high-fat diet and fimasartan. (a) Fat tissue slides of WKY rats and SHRs were fixed and stained by hematoxylin and eosin reagents. Magnification is 100x. (b) The number of adipocytes per unit square (5461.06 μm²) in slides stained by hematoxylin and eosin reagents. (c) The concentration of adiponectin in visceral fat extracts of WKY rats and SHRs was estimated with adiponectin colorimetric assay kit. The results are expressed as means ± SEM (n = 3 or 6). Values were statistically analyzed by unpaired t-test. All experiments were repeated three times. Meaning of indications: WKY means normal control rat given normal diet containing 10% fat, S10 means SHRs given normal diet containing 10% fat, S10F means SHRs given normal diet containing 10% fat plus fimasartan, S60 means SHRs given high-fat diet containing 60% fat, and S60F means SHRs given high-fat diet containing 60% fat plus fimasartan.