Figure 4. Simvastatin-induced cell death in glioblastoma, non-small lung cancer cell, and breast cancer cell lines dependents on FPP and GGPP.

7.5, 15 μM FPP, or 7.5, 15 μM GGPP were added to the cells 4 hrs prior to treatment with simvastatin (10 μM, 96 hrs) on cell death, measured by MTT assay in U87 (A,B), A549 (C,D), and MDA-MB-231 (E,F). For each experiment control cells were treated with simvastatin solvent (DMSO) alone (control) or with both DMSO and the appropriate solvent for each cholesterol precursor (i.e. DMSO for “FPP control” and “GGPP control”). Results are expressed as mean ± SD of 9 replicate in 3 independent experiments (*P < 0.05, **P < 0.01, and ***P < 0.001).