Figure 4. ABLs carrying PA or PI5P promote intracellular bacterial killing in primary macrophages expressing a pharmacologically inhibited CFTR.

Monocyte derived macrophages (MDM) isolated from healthy subjects (n = 8) were infected with P. aeruginosa, as described in materials and methods, and then treated for 2 hours with the ABLs carrying PA, PI3P or PI5P in the absence (a–c) or in the presence (d–f) or of the CFTR inhibitor INH172. Bacterial growth was assessed by CFU assay. The results shown are obtained from triplicate for each condition. Statistical analysis was performed by using two sided Wilcoxon matched-pairs signed rank test and p value is indicated in the single panels.