Figure 6. Chromium picolinate in vivo inhibits TSP-1, OGT, PCNA and Vimentin expression, increases SM-MHC expression and reduces protein O-glycosylation in aortic lysates of STZ-induced hyperglycemic ApoE^−/− mice.

Shown are representative immunoblots depicting (a) TSP-1, (b) O-GlcNAc, (c) OGT, (d) PCNA, (e) SM-MHC expression (upper panel) and vimentin expression (middle panel). Membranes were probed with anti-β-actin used as loading control. In each case, graphs represent summary data for densitometric quantification of immunoblots (at least 3 mice per group). For c and e, lane images show proteins detected on a single immunoblot; however, lanes were rearranged for clarity of presentation; the corresponding original uncropped blots are presented in Supplementary Fig. S7. All results are presented as fold of Control; values are expressed as means ± SD; ^*p ≤ 0.05 vs. Control; ^#p ≤ 0.05 vs. STZ.