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. 2017 Mar 27;7:45275. doi: 10.1038/srep45275

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Copyright © 2017, The Author(s)

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(a) The expression of LOX-1 in various gastric cancer cell lines were detected by western blotting. (b) The expression of LOX-1 in MGC80-3 cells transfected with vector or LOX-1 overexpressing plasmid (Left panel) and AGS cells stably expressing LOX-1-specific shRNA (Right panel) were detected by western blotting. (c) Transwell migration and matrigel invasion assays were conducted in MGC80-3 cells transfected with vector or LOX-1. Data are mean ± SD. (d) Transwell migration and matrigel invasion assays were conducted in AGS cells stably expressed control shRNA (shCT) or two LOX-1-specific shRNA (sh1/sh2). Data are mean ± SD. (e,f) The expression of epithelial markers ZO-1 and E-cadherin, mesenchymal marker Vimentin and transcriptional repressors Snail and Twist were detected by western blotting in LOX-1-overexpressing MGC80-3 cells (e) and LOX-1-knock-down AGS cells (f).