Figure 4. Sequential dαN(i, i + 1) nuclear Overhauser effect (NOE) connectivities for refolded rattusin (r-RTSN) in a ¹H 2D NOESY spectrum observed with a mixing time of 200 ms at 298 K.

Intra-residue NH-C^αH cross peaks are labeled with the residue number of RTSN using standard single-letter amino acid abbreviations. The first residue of RTSN was not observed in the NOESY spectrum.