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(a) The two C9ORF135 isoforms in H1 and H9 hESCs. GAPDH served as an internal control. (b) C9ORF135 expression in various cell lines and tissues. (c) RT-qPCR analysis of C9ORF135 expression during H1 hESC differentiation. (d) C9ORF135 gene structure and its two isoforms. C9ORF135 consists of six exons. Isoform 1 includes exons 1 and 2, whereas isoform 2 includes exon 1 and 3. The junction of exons 1 and 3 form a “TGA” stop codon.
