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. 2017 Mar 27;7:45311. doi: 10.1038/srep45311

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Ectopic C9ORF135 expression in HEK293 cells. C9ORF135 (left, red); DAPI (middle, blue); Merge (right). Scale bars, 25 μm. (b) Localization of exogenous C9ORF135 in mouse E14 cells. Scale bars, 25 μm. (c) Localization of endogenous C9ORF135 protein in hESCs. (d) Flow cytometry analysis for hESCs stained with C9ORF135 antibody. (e) C9ORF135 and E-cadherin immunofluorescence in hESCs. Scale bars, 25 μm. A typical result from triplicate experiments is shown. (f) Subcellular fractionation and western blot analysis of hESCs. Lanes from left to right: hESC, total lysate; M, protein marker; 1, plasma membrane; 2, nucleus; 3, cytoskeleton (short lysis); 4, cytoskeleton (prolonged lysis); 5, cytoplasm; 6, HEK293A negative control cells. A typical result from triplicate experiments is shown.

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