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. 2017 Mar 27;7:45311. doi: 10.1038/srep45311

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Chromatin immunoprecipitation (ChIP) analysis was conducted using SOX2 and OCT4 antibodies and C9ORF135-specific primers. (b) C9ORF135 promoter activity in the presence or absence of OCT4 and SOX2. (c) OCT4, NANOG, and SOX2 expression analysis in differentiated H1 hESCs. (d) C9ORF135 expression analysis in H1, H9, and LiY ESCs differentiated with retinoic acid (RA) for 7 days. The result shown is the average of triplicate experiments.

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