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. 2017 Mar 27;7:45180. doi: 10.1038/srep45180

Figure 2. Activation of the MS ion channel MscL-G22S in DHBs.

Figure 2

(a) Schematic showing the activation strategy. A nanoinjector equipped with a pulled glass pipette was inserted into the droplet (left). The injection of nanolitre volumes of buffer caused the droplet monolayer to stretch (right) thereby inducing an asymmetric tension in the DHB (red arrows), thus activating the channel. (b) Transmission micrograph of the bilayer viewed from the top. The inner white ring is the periphery of the bilayer and the larger black ring is the periphery of the droplet. An electrode (black) and nanoinjector (indicated by white dotted lines) were inserted into the droplet. The scale bar is 100 μm. (c) Current traces, recorded at +30 mV in DHBs containing MscL-G22S, upon injection of 5 nL (left), 18 nL (middle) and 23 nL (right) experiment buffer. (d) Current traces recorded at +100 mV in DHBs containing resting or closed KcsA in a neutral pH buffer (pH = 7.4), upon the injection of 23 nL experiment buffer.