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. 2017 Mar 27;7:45181. doi: 10.1038/srep45181

Figure 4. Npnt induced cell proliferation in ex vivo organ cultures and in vitro.

Figure 4

(A) E13 tooth germs were transfected with control or Npnt siRNA and cultured for 8 days. (B) Relative tooth size plot (n = 10), with average tooth germ size in control siRNA group set at 1.0. (C) Cell proliferation was analyzed using a CCK-8 assay after coating the dishes with recombinant Npnt or transfection with Npnt. BrdU incorporation of M3H1 cells after coating dishes with recombinant Npnt or transfection with Npnt. The ratio was calculated as BrdU-positive cells/DAPI-stained nuclei. (D) Western blotting results of cyclin D1, Sox2, V5, and Gapdh in M3H1 cells transfected with mock vector or Npnt expression vector. Gapdh was used as the internal control. The full-length blots are presented in Supplementary Figure 1A. *P < 0.05. Error bars represent mean ± S.D. Scale bars: 100 μm.