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. 2017 Mar 27;7:45181. doi: 10.1038/srep45181

Figure 7. EGF-like repeats of Npnt regulate EGF signaling pathway by regulating phosphorylation of Akt.

Figure 7

(A) Western blotting of P-Akt, Akt, PI3K, P-PLCγ, P-Stat5, P-Erk1/2, Erk1/2, and Gapdh in M3H1 cells transfected with Npnt-FL or Npnt-ΔEGF, and cultured for 48 hours. Gapdh was used as the internal control. The full-length blots are presented in Supplementary Figure 1B. (B) Western blotting of P-Akt and Akt in M3H1 cells with or without recombinant Npnt treatment for 60 minutes. Gapdh was used as the internal control. The full-length blots are presented in Supplementary Figure 1C. (C) Ratio of Sox2+ cells among M3H1 cells transfected with Npnt-FL and treated with different doses of PI3K inhibitor (LY294002). The ratio was calculated as Sox2 positive cells/DAPI-stained nuclei. (D) BrdU incorporation in M3H1 cells treated with different doses of LY294002. The ratio was calculated as BrdU-positive cells/DAPI-stained nuclei. *P < 0.05. Error bars represent mean ± S.D.