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. 2017 Mar 27;7:45379. doi: 10.1038/srep45379

Figure 5. PTPIP51 increased mitochondria-SR contacts in cardiomyocytes.

Figure 5

(A) Confocal images of Ad-lacZ or Ad-PTPIP51 transfected cardiomyocytes loaded with Mito-Tracker Green and ER-Tracker Red. Bar charts are mean intensity correlation quotients (ICQ) (left) and Pearson correlation coefficient (PCC) (right). n = 59–61 frames in each group from three independent experiments. Scale bars: 7 μm and 3.5 μm (Merge). Green: Mito-Tracker green; Red: ER-Tracker red; ICA: intensity correlation of green and red fluorescent signals and high correlation is displayed in yellow. (B) TEM images of mitochondria-SR contacts in neonatal cardiomyocytes as indicated. Red lines with arrows indicate mitochondria-SR contacts. Scale bar: 500 nm. Yellow square areas in the Ad-PTPIP51 cell were further zoomed in to show the tight contacts of mitochondria (Mito) and SR (arrowheads). Scale bars: 1 μm, 200 nm (zoom upper), and 40 nm (zoom bottom). Bar charts are % of the mitochondrial surface closely in contact with SR (upper) and mean distance between mitochondria and SR junction (bottom). n = 37–50 frames in each group from four independent experiments. *P < 0.05 versus Ad-lacZ. (C) Confocal images of scramble or PTPIP51 shRNA-transfected cardiomyocytes loaded with Mito-Tracker Green and ER-Tracker Red. Bar charts are mean ICQ (left) and PCC (right). n = 69 frames in each group from three independent experiments. Scale bars: 7 μm and 3.5 μm (Merge). (D) TEM images of mitochondria-SR contacts in neonatal cardiomyocytes as indicated. Red lines are mitochondria-SR contacts. Scale bar: 500 nm. Low-magnification TEM image of PTPIP51 shRNA-transfected cardiomyocytes (right). Scale bar: 1 μm. Bar charts are % of the mitochondrial surface closely in contact with SR (upper) and mean distance between mitochondria and SR junction (bottom). n = 43–45 frames in each group from four independent experiments. *P < 0.05 versus scramble group.