. 2017 Mar 27;7:45403. doi: 10.1038/srep45403

Table 5. Effect of ouabain and K⁺-free medium on intracellular Na⁺ and the content of mRNA encoding EGR1, PTGS2, and ATF3.

Incubation medium	Na⁺_i content, nmol/mg protein	mRNA content, relative units
Incubation medium	Na⁺_i content, nmol/mg protein	EGR1	PTGS2	ATF3
Control (DMEM)	84.0 ± 12.3	1.00	1.00	1.00
Ouabain, 100 nM	511 ± 52	7.34 ± 0.20	5.96 ± 0.09	2.14 ± 0.07
K⁺-free DMEM	843 ± 77	8.16 ± 0.17	6.51 ± 0.11	3.17 ± 0.16

Cells were incubated in control and K⁺-free medium as well as in the presence of 100 nM ouabain for 6 h. mRNA content was estimated by RT-qPCR and was taken in control medium as 1.00. Means ± S.E. from four experiments performed in triplicates (Na⁺ content) and duplicates (mRNA content) are shown.

Table 5. Effect of ouabain and K+-free medium on intracellular Na+ and the content of mRNA encoding EGR1, PTGS2, and ATF3.

Table 5. Effect of ouabain and K⁺-free medium on intracellular Na⁺ and the content of mRNA encoding EGR1, PTGS2, and ATF3.