Fig. 3.

In vitro reactive nitrogen species (RNS) scavenging activities and iron chelating capabilities of TBHE, TBCE, TBEE, TBBE and TBWE together with reference compounds. a nitric oxide inhibition compared to standard curcumin, TBHE (2, 7 µg/ml) have a significance level of p < 0.01, rest of the values are highly significant (p < 0.001). All values are compared to control (0 μg/ml), b peroxynitrite radical scavenging compared to standard gallic acid, TBHE (20, 100 µg/ml) has a significance level of p < 0.05, TBCE (20, 50, 100 µg/ml), TBBE (20 µg/ml), TBWE (50 µg/ml) have p value of < 0.01, rest of the values have p value < 0.001. All values are compared to control (0 μg/ml), c iron chelation activity of extracts, all the values are highly significant (p < 0.001 vs control i.e. 0 µg/ml) and d of standard iron chelator EDTA. The results are mean ± S.D. of six parallel measurements. Cur curcumin, GA gallic acid, EDTA Ethylenediamine tetra acetic acid