Table 1.
IC50 values of all fractions of T. belerica for antioxidant and cytotoxicity assays
| TBHE | TBCE | TBEE | TBBE | TBWE | Standard | Values of standard compounds | |
|---|---|---|---|---|---|---|---|
| TEAC value | 0.002 ± 0.001 | 0.604 ± 0.003 | 0.674 ± 0.011 | 0.984 ± 0.003 | 0.954 ± 0.006 | Trolox | 1 |
| IC 50 values of the extracts for free radical scavenging capacity for | |||||||
| Reactive oxygen species (ROS) scavenging assays | |||||||
| DPPH | 4054.95 ± 807.27 | 21.89 ± 0.43 | 7.11 ± 0.31 | 17.20 ± 0.13 | 9.51 ± 0.07 | Ascorbic acid | 5.29 ± 0.28 |
| Hydroxyl (OH−) | 25.93 ± 0.61 | 27.3 ± 0.65 | 21.57 ± 0.54 | 19.42 ± 0.57 | 614.67 ± 18.57 | Mannitol | 571.45 ± 20.12 |
| Hypochlorous acid (HOCl) | 2151.49 ± 400.91 | 451.97 ± 16.83 | 615.88 ± 68.48 | 1382.26 ± 198.44 | 620.69 ± 33.05 | Ascorbic acid | 235.96 ± 5.75 |
| Superoxide (O2 −) | 902.13 ± 52.1 | 322.09 ± 7.37 | 18.77 ± 0.28 | 24.05 ± 0.44 | 21.76 ± 0.54 | Quercetin | 42.06 ± 1.35 |
| Singlet (1O2) | 5684.66 ± 1501.94 | 6121.94 ± 966.73 | 8771.79 ± 2062.24 | 5717.27 ± 976.09 | 3270.87 ± 151.44 | Lipoic acid | 46.15 ± 1.16 |
| Hydrogen peroxide (H2O2) | 20.79 ± 1.35 | 10.29 ± 0.75 | 14.62 ± 1.14 | 17.46 ± 1.62 | 31.05 ± 6.82 | Sodium pyruvate | 3.24 ± 0.3 |
| Reactive nitrogen species (RNS) scavenging assays | |||||||
| Nitric oxide (NO) | 1665.39 ± 202.5 | 134.05 ± 2.23 | 40.44 ± 0.87 | 64.93 ± 0.96 | 16.71 ± 0.23 | Curcumin | 90.82 ± 4.75 |
| Peroxynitritre (ONOO−) | 4784.85 ± 279.08 | 3751.96 ± 145.46 | 1369.81 ± 55.88 | 1092.26 ± 87.02 | 2366.85 ± 306.94 | Gallic acid | 876.24 ± 56.96 |
| Fe chelation | 1094.05 ± 78.26 | 112.40 ± 1.36 | 13.22 ± 0.17 | 18.82 ± 0.61 | 9.49 ± 0.47 | Ethylene di-amine tetra acetic acid | 1.27 ± 0.05 |
| IC 50 values of cytotoxicity of T. belerica fruit fractions against various malignant and normal cell lines | |||||||
| Cell lines | |||||||
| A549 | 2258.78 ± 1157.06 | 1260.5 ± 101.2 | 1478.02 ± 227.52 | 1283.83 ± 115.65 | 1394.33 ± 556.19 | ||
| MCF-7 | 1052.02 ± 188.01 | 204.35 ± 9.91 | 115.90 ± 18.97 | 179.99 ± 0.94 | 122.91 ± 0.58 | ||
| HeLa | 6122.41 ± 1552.33 | 664.79 ± 35.98 | 299.09 ± 16.23 | 389.39 ± 12.74 | 257.56 ± 5.08 | ||
| HepG2 | 1339.93 ± 123.95 | 1157.42 ± 245.31 | 1644.14 ± 287.65 | 1229.53 ± 140.93 | 516.95 ± 66.57 | ||
| U87 | 1208.98 ± 178.86 | 221.97 ± 10.31 | 36.52 ± 1.76 | 20.13 ± 0.93 | 17.27 ± 0.31 | ||
| WI-38 | 1950.72 ± 388.17 | 1805.61 ± 368.25 | 1277.23 ± 15.41 | 494.32 ± 6.87 | 872.07 ± 22.10 | ||
IC50 values of all activities are determined in μg/ml. Data are expressed as mean ± S.D. (n = 6)