Fig. 1.

LiCl promoted the proliferation of rat NSCs. The ratio of Ki67 positive cells in LiCl-treated NSCs (a) was higher than that in the untreated group (b). c Statistical analysis of Ki67⁺ cells ratio with the treatment of LiCl for 1-3 days. d Ratio of Ki67⁺ cells in NSCs was raised with the increasing concentration of LiCl. e Statistical analysis showed that the ratio of BrdU⁺ cells was increased in the LiCl-treated NSCs. Statistical difference was determined by unpaired Student’s t tests. f The mRNA expression level of GSK-3β, β-catenin and Cyclin D1 was altered in the presence of LiCl. *p < 0.05. Scale bar 20 μm