Table 1.
AHL-based biosensors used for the identification of active QSI molecules from the sea.
| Strain/Plasmid | QS System | Reporter System | Detection Range | References |
|---|---|---|---|---|
| Chromobacterium violaceum CV026 | CviI/R | Violacein pigment | C6-HSL, 3-oxo-C6-HSL, C8-HSL, 3-oxo-C8-HSL | [35,36,37,38] |
| Chromobacterium violaceum CV017 | CviI/R | Violacein pigment | 3-oxo-C6-HSL, C8-HSL, 3-oxo-C8-HSL | [39,40,41] |
| Escherichia coli pSB403 | LuxI/R (V. fisheri) | luxCDABE | 3-oxo-C6-HSL, C6-HSL, 3-oxo-C8-HSL, C8-HSL | [37,42] |
| Escherichia coli pSB536 | AhyI/R (A. hydrophyla) | luxCDABE | C4-HSL | [24] |
| Escherichia coli pSB401 | LuxI/R (V. fisheri) | luxCDABE | 3-oxo-C6-HSL, C6-HSL, 3-oxo-C8-HSL, C8-HSL | [39,40,43,44,45,46,47] |
| Escherichia coli pSB1075 | LasI/R (P. aeruginosa) | luxCDABE | 3-oxo-C12-HSL, 3-oxo-C10-HSL, C12-HSL | [39,40,41] |
| QSIS2 | LasI/R (P. aeruginosa) | luxCDABE | 3-oxo-C12-HSL, 3-oxo-C10-HSL, C12-HSL | [25] |
| Vibrio harveyi JMH 612 | LuxPQ (Vibrio harveyi) | luxQ | 3-OH-C4-HSL | [48,49] |
| Agrobacterium tumifaciencens pZLR4 | TraI/R (A. tumefaciens) | β-galactosidase | All 3-oxo-HSLs | [41,50,51] |
| Escherichia coli pKDT17 | LasI/R (P. aeruginosa) | β-galactosidase | 3-oxo-C12-HSL, C12-HSL, C10-HSL, 3-oxo-C10-HSL | |
| QSIS1 | LuxI/R (V. fisheri) | β-galactosidase | 3-oxo-C6-HSL, C6-HSL, C8-HSL, C10-HSL | [25] |
| pAS-C8 | CepI/R (B. cepacia) | gfp | C8-HSL | [42] |
| pKR-C12 | LasI/R (P. aeruginosa) | gfp | 3-oxo-C12-HSL, 3-oxo-C10-HSL | [42] |
| Escherichia coli JB525 | LuxI/R (V. fisheri) | gfp | 3-oxo-C6-HSL, C6-HSL, C8-HSL, C10-HSL | [35] |
| QSIS3 | LuxI/R (V. fisheri) | gfp | 3-oxo-C6-HSL, C6-HSL, C8-HSL, C10-HSL | [25] |
| Tn5-Las | LasI/R (P. aeruginosa) | gfp | 3-oxo-C12-HSL, 3-oxo-C10-HSL | [25] |