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. 2017 Feb 25;15(3):56. doi: 10.3390/md15030056

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Schema showing the life history and changes in TTX content of the flatworm Planocera multitentaculata. The flatworm lays eggs underneath stones during the spawning period. The body weight/size decreased in association with time during the spawning period. Recruitment of the flatworm was sequentially observed during the spawning period (April–July). Hatched larvae settled and individuals of the new generation were found in August–September. These newly settled individuals had a high level of TTX.