Analyses (by ELISA) of the inhibitory effect of KO emulsion on the TNF-α release by LPS-EB differentiated human THP-1 macrophages. (A) LPS-EB 0.01 µg/mL, or (B) 0.1 µg/mL was co-incubated overnight together with different concentrations of KO or 10 µg/mL LPS antagonist (LPS-RS-AN) and afterwards for 3 h with the differentiated THP-1 macrophages. Values (in %TNF-α release relative to positive (LPS-EB) control (=100% release)) are given as the mean + SEM; ANOVA test, significance vs. positive control (=LPS-EB), * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001; n = 3–5 independent experiments.