Figure 8.
The effect of 4-2A on the expression of neurotrophin genes: NGF and BDNF. NGF mRNA expression (A); and protein expression (C); and BDNF mRNA expression (B); and protein expression (D), normalized to the expression of housekeeping gene β-Actin, in differentiated SH-SY5Y cells treated with Aβ25–35 (20 μM) and/or 4-2A (10 μg/mL) for 4, 8, 12, 24 h. The data of both mRNA and protein expression from six independent experiments (n = 1 in each experiment) were analyzed and expressed as mean ± SEM. ** p < 0.01 vs. control group; # p < 0.05, ## p < 0.01 vs. Aβ25–35 group.