Figure 1.

(A) Proposed model for reversible m⁶A methylation. m⁶A on RRACH is mainly appended by the METTL3-METTL14 complex, and oxidative demethylation is performed by m⁶A demethylase. CCCH: Cys-Cys-Cys-His type zinc finger motif (ZnF). (B) Schematic illustration of METTL3 and METTL14 domain structures. The METTL3 ZnF and METTL3 methyltransferase (abbreviation as MTase) domains are colored in magenta, and the METTL14 methyltransferase domain is colored in cyan. Detailed domain boundaries are labeled beneath the structures. (C) Comparison of the methyltransferase activity of full-length (abbreviation as FL) and truncated methyltransferase complexes. The c.p.m. of the extracted RNA was measured in a scintillation counter and averaged ( ± s.e.m.); the c.p.m. was determined from 3 replicates.