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. 2017 Jan 13;14(3):347–360. doi: 10.1080/15476286.2017.1279786

Figure 3.

Figure 3.

MiR-432 inhibited myoblast proliferation. MiR-432 mimics or negative control (NC) were transfected into cells at 50% density at 50nM and cells were harvested on 24 h after transfection. (A) The overexpression efficiency of miR-432 after transfecting miR-432 mimics compared with negative control (NC). (B) Real-time qPCR was used to detect cell cycle genes, Cyclin E, cdk2 and PCNA after 24 h transfection. (C) Western blot analysis of cell cycle genes. (D) Cell cycle analysis were performed by flow cytometer after transfection for 24 h. (E) The statistics results of cell cycle analysis. (F) EdU assay was carried out after transfection for 24 h. Cells during DNA replication were stained by EdU (red) and cell nuclei were stained with Hoechst (blue). (G) The percentage of EdU positive cells / Hoechst positive cells was quantified. (H) Cell count was measured by cell count kit 8 (CCK8), results represented absorbance value at 490 nm after incubation with 10% CCK8 solution for 4 h. Data were representative of means ± SD of three independent experiments.*, P < 0.05;**, P < 0.01.