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. 2017 Jan 13;14(3):347–360. doi: 10.1080/15476286.2017.1279786

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Figure 5. — MiR-432 suppressed myoblast differentiation. MiR-432 mimics or negative control (NC) were transfected into cells at 70% density at 50nM. The total RNA on day 0, 2, 4 for RT qPCR and protein on day 4 for western blotting analysis. (A) The overexpression efficiency of miR-432 after transfecting miR-432 mimics compared with negative control (NC). (B, C) Real-time qPCR was used to detect cell myogenic genes, MyoG, MyHC (MYH1) after inducing. (D) Immunofluorescence of muscle myosin heavy chain (MyHC) in C2C12 cells on fourth day of differentiation. (E) The fusion index was counted by MyHC-positive cells to total nuclei (Hoechst-positive cells). (F) Western blot analysis of myogenic genes on fourth day of differentiation. (G) The quantify results of protein level. Data were representative of means ± SD of three independent experiments. *, P < 0.05; **, P < 0.01.