Figure 2.
Excised-patch clamp reveals functional properties of KATP channels in zebrafish β-cells. (a) Individual KATP channels (4.35 pA at −50 mV) are detected in three representative membrane patches excised from zebrafish β-cells. (b) These K+ currents are inhibited by ATP, with (c) IC50 = 22.6 mM, nH = 1.01. (d) These channels are also activated by increasing [Mg-ADP], quantified in (e). ****p < 0.0001 (Mann–Whitney test). The dose–response curve was generated from 16 cells derived from six pools of zebrafish islet (biological replicates). The ADP response graph comprises nine cells derived from four biological replicates. (a,b and d) Representative traces.