Figure 5.

Effect of lysozyme on Candida albicans ATCC 10231 biofilm cohesion. (A) C. albicans ATCC 10231 cells were cultivated for 24 h at 37°C and subsequently washed three times with sterile 0.9% NaCl. Absorbance at 600 nm was measured at each step of the washing process. Black columns represent yeast suspensions following 24 h cultivation at 37°C; white columns represent the control, where wells were measured from fresh suspension in liquid Sabouraud following no incubation. (B) Absorbance at 600 nm after each wash was measured in yeast cultured in 0, 10 or 1,000 µg/ml of lysozyme (n=4 independent experiments). Data are expressed as the mean ± standard error of the percentage relative to the absorbance of the initial supernatant (n=4 independent experiments, 8 technical replicates per condition).