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Primer information.
| Gene | Sequence (5′-3′) | Tm (°C) | Production length (bp) |
|---|---|---|---|
| IL-6 | Forward: CCACTTCACAAGTCGGAGGCTTA | 78 | 169 |
| Reverse: CCAGTTTGGTAGCATCCATCATTTC | |||
| TNF-α | Forward: TATGGCCCAGACCCTCACA | 80.6 | 199 |
| Reverse: GGAGTAGACAAGGTACAACCCATC | |||
| GAPDH | Forward: AAATGGTGAAGGTCGGTGTGAAC | 75.6 | 90 |
| Reverse: CAACAATCTCCACTTTGCCACTG |
IL-6, interleukin-6; TNF-α, tumor necrosis factor-α.
