Figure 5.

RA-induced apoptosis by curcumin in TNBC cells is regulated by CRABPII/RAR pathway. (A) MDA-MB-231 and (B) MDA-MB-468 cells were transfected with CRABPII or control siRNA for 24 h, then treated with 10 µM curcumin in the presence or absence of 1 µM ATRA (RA) for 96 h, protein extracted and expression level of caspase-9 was probed with antibodies recognizing caspase-9. GAPDH was used as a loading control. TNBC, triple-negative breast cancer; CRABPII, cellular retinoic acid-binding protein II.