Figure 4.

Physiological testosterone inhibits AGE-induced JAK2/STAT3 pathway activation. Representative western blot images and quantification of p-JAK2 and p-STAT3 in human umbilical vein endothelial cells following 48 h exposure to AGEs with or without 30 nM testosterone and normalized against total protein. Unmodified BSA, AGEs or 30 nM testosterone. Data are shown as the mean ± standard error of the mean of triplicate experiments. *P<0.05, vs. BSA; ^#P<0.05, vs. AGEs. AGEs, advanced glycation end product; JAK2, Janus kinase 2; STAT3, signal transducer and activator of transcription 3; p-phosphorylated; t-, total; BSA, bovine serum albumin; T, testosterone.