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. 2017 Jan 26;15(3):1326–1334. doi: 10.3892/mmr.2017.6149

Figure 3.

Figure 3.

miR-410 directly targets ATG161L in osteosarcoma cell lines. (A) miR-410/ATG16L1 alignment by miRanda analysis, and schematic diagram of the pMIR-ATG16L1/pMIR- ATG16L1mut paired sequences for miR-410. (B) Normalized luciferase activity of pMIR-ATG16L1/pMIR-ATG16L1mu reporter in human embryonic kidney 293T cells transfected with miR-410 or NC mimics. *P<0.05. (C) Immunofluorescent detection of ATG16L1 expression in U2OS and MG-63 cells transfected with miR-410 or NC for 48 h. (D) Western blotting was conducted to detect the effects of miR-410 on ATG16L1 expression post-transfection with miR-410 or NC for 48 h. Rapamycin (20 nM), which is an activator of autophagy, increased ATG16L1 expression. Data are presented as the mean ± standard deviation; *P<0.05. ATG161L, autophagy related 16-like 1; miR-410, microRNA-410; NC, negative control; WT, wild-type; mut/MT, mutant; Rap, rapamycin; UTR, untranslated region; DAPI, 4′,6-diamidino-2-phenylindole.