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. 2017 Jan 5;15(3):1211–1221. doi: 10.3892/mmr.2017.6104

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Copyright: © Guo et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — miR-21 expression is upregulated in HS. (A) The expression of miR-21 was analyzed by RT-qPCR and normalized to U6 snRNA in 10 pairs of NS and HS tissue samples. (B) The expression of miR-21 in 10 paired NSFB and HSFB samples were analyzed by RT-qPCR. (C) ISH was performed to determine the localization and expression of miR-21 in skin tissue of HS or NS samples. ISH of skin tissues highlighted extensive cytoplasmic staining for miR-21 (red), which localized in epithelial cells and subdermal fibroblasts. The expression of miR-21 in HS tissues was higher compared with NS controls. Bars: 20 µm. miR, microRNA; HS, hypertrophic scar; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NS, normal skin; NSFBs, normal skin fibroblasts; HSFBs, hypertrophic scar fibroblasts; ISH, in situ hybridization; snRNA, small nuclear RNA. **P<0.01.