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. 2017 Jan 5;15(3):1279–1285. doi: 10.3892/mmr.2017.6105

Figure 1.

Figure 1.

Silencing of S100A6 downregulated the mRNA and protein expression levels of β-catenin in eutopic endometrial stromal cells, as determined by RT-PCR and western blot analysis. (A) Western blot analysis indicated decreased S100A6 and β-catenin protein levels in eutopic endometrial stromal cells following treatment with Lv-siS100A6 compared with Lv-control. Lane 1, untreated group; lane 2, Lv-control group; lane 3, Lv-siS100A6 group. The effect of siS100A6 on S100A6 (B) protein and (C) mRNA expression, and β-catenin (D) protein and (E) mRNA expression was evaluated 4 days post-transfection by western blotting and RT-PCR, respectively. Data are presented as the mean ± standard deviation. *P<0.05 vs. untreated and Lv-control groups. S100A6, S100 calcium binding protein A6; si, small interfering RNA; Lv-siS100A6, siS100A6 recombinant lentivirus vector; Lv-control, control recombinant lentivirus vector; RT-PCR, reverse transcription-polymerase chain reaction.