Figure 2.

Parasite gene copy numbers (per microliter) detected by SYBR Green (Thermo Scientific, Foster City, CA, USA) quantitative PCR and parasitemia (parasites per microliter) determined by microscopy of Plasmodium malariae samples from asymptomatic and symptomatic persons. Median, first quartile, and fourth quartile of the data are shown for each sample category (horizontal lines). No significant difference was observed between asymptomatic and symptomatic persons in terms of P. malariae parasite gene copy number and parasitemia. Squares represent samples with gene copy number measured by quantitative PCR; circles, samples with parasitemia estimated by microscopy; closed squares and circles, P. malariae samples from asymptomatic persons; open squares and circles, P. malariae samples from symptomatic patients. NS, not significant.