FIGURE 6.
Topical application of BMV109 on human carotid endarterectomy sample. (A) Macroscopic specimen. (B) SDS-PAGE analysis followed by flatbed laser scanning to detect probe-labeled cathepsins in carotid artery tissue lysates treated with or without cathepsin inhibitor GB-111NH2. (C) Fresh frozen tissue cross-sections of human carotid artery were labeled with optical probe BMV109 (red) and costained with macrophage activation marker CD68 (green), elastin (yellow), and cathepsin S (cyan). DAPI nuclear stain is shown in blue. Samples were tile-scanned at high resolution to generate full images for which scale bar represents 1 mm. White boxes on full images indicate region that higher magnification images were taken at 40×. Scale bars on zoom images are 10 μm.