FIGURE 1.

Strategies to evaluate the viability of nonreplicating mycobacteria for high-throughput screening. The arrow color indicates the quality of each readout strategy (considering robustness, ease of use, dynamic range, etc.) as excellent (green arrows), average to poor (black arrows), or infeasible (red line). Compound carryover may result from compound transfer from the nonreplicating assay to replicating assay bacteriologic growth medium or by compound adherence to the bacterial cell wall.