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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Immunol. 2017 Jan 18;198(5):2182–2190. doi: 10.4049/jimmunol.1601480

Figure 3. Transcription factor control of Th17 and Th22 cell differentiation.

Figure 3

Representative plots of WT, Rorc(γt)-/- and Tbx21-/- Th cells differentiated in each Th condition (Th1, Th17 and Th22) (A). Cell populations in FACS plots are pre-gated on CD4+CD44+ and viable cells. Quantitation of IL-17+, IL-22+ and IFN-γ+ cells under each Th condition (Th1, Th17 and Th22) for WT, Rorc(γt)-/- and Tbx21-/- cells (B) and differential transcription factor expression assessed by RT-PCR, normalized to the housekeeping gene Hprt (C). Error bars represent SEM (n=6 per group from three independent experiments). **p < 0.01, ***p < 0.001, ****p < 0.0001.