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Diagram of the production of proteoliposomes in a cell-free expression system.
Notes: Cell-free transcription and translation are initiated by adding liposomes and DNA plasmid into the reaction medium. This system also requires E. coli extract, an energy regeneration system and substrates (NTPs, salts, amino acids, chaperones, cofactors, etc).
Abbreviations: E. coli, Escherichia coli; NTPs, nucleotide triphosphates.
