Figure 4.

In vitro NADPH oxidase activity of NOX2/p22^phox and negative liposomes.

Notes: (A) Representative results of the NADPH oxidase activity of NOX2/p22^phox (8 pmol cytochrome b₅₅₈) and negative liposomes in a cell-free system assay in the presence of the recombinant p47^phox, p67^phox and Rac (1 µM), arachidonic acid (400 µM) and NBT (100 µM), stimulated or not with NADPH (200 µM). (B) NADPH oxidase activity was expressed in moles of superoxide O₂^•− produced/s/mol of heme and measured before (total activity) or after SOD addition (SOD non-inhibitable activity). Cell-free system assay was performed in the same experimental conditions as (A).

Abbreviations: DPI, diphenyleneiodonium; NADPH, nicotinamide adenine dinucleotide phosphate; NBT, nitroblue tetrazolium; OD, optical density; O₂^•−, superoxide anion; SOD, superoxide dismutase; PLs, proteoliposomes.