Figure 7.

Analysis of in cellulo toxicity and NADPH oxidase activity restoration in X⁰-CGD iPSC-derived macrophages.

Notes: (A) Viability of CGDX⁰ iPSC-derived macrophages after 8, 12 and 24 h of treatment. Untreated cells are used as positive control (100%) and DMSO-treated cells as negative control. Data are expressed as mean ± SEM (n=3). The differences of viability between the untreated cells and the liposome-treated cells were analyzed using the nonparametric Mann–Whitney test. (B) Morphological images of X⁰-CGD iPSC-derived macrophages after 8, 12 and 24 h of treatment with NOX2/p22^phox liposomes or incubated with IMDM only (magnification ×10). (C) X⁰-CGD macrophages were treated for 8 h with NOX2/p22^phox or negative liposomes. Then, WT and X⁰-CGD macrophages were stimulated with PMA (resting; scale bars =50 µm).

Abbreviations: CGD, chronic granulomatous disease; DMSO, dimethylsulfoxide; IMDM, Iscove’s Modified Dulbecco’s Medium; iPSC, induced pluripotent stem cell; NADPH, nicotinamide adenine dinucleotide phosphate; ns, nonsignificant; PMA, phorbolmyristate-acetate; SEM, standard error of the mean; WT, wild type; X⁰-CGD, X⁰-linked CGD.