Figure 5.

MALAT1 controls the expression of cyclin-dependent kinase (CDK) inhibitors in vascular smooth muscle cells. (a) Upon transfection with GapmeR targeting MALAT1, the mRNA levels of all relevant CDK inhibitors were found to be significantly changed compared to GapmeR negative control transfected cells (n = 5). (b) Additionally, the observed mRNA levels of the genes CDKN1A (p21), CDKN1B (p27), and CDKN2C (p18) were confirmed on the protein level by Western blotting (n = 5). Statistical analysis by unpaired (a and b) student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001)