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. 2017 Mar 27;12(3):e0174550. doi: 10.1371/journal.pone.0174550

Fig 4. gp120 induces neutrophils and lymphocytes migration but inhibits monocytes migration.

Fig 4

Transwell immune cells migration analyzed with evaluation of PBMC subpopulation counts by flow cytometry. 2*10^5 cells were incubated with gp120 mutants and then cultured in insert with a 3μm membrane. Active migration of cells was evaluated after harvest of the medium in the wells. Cells were stained with anti CD15, CD14 and CD4 antibodies and counts were normalized to 1000 beads. Statistic analysis were performed with variance test ANOVA and then with a t-tests to compare positive conditions to the mock control (* p<0.05 and ** p<0.01).