Table 1. Human gene RT-qPCR primers list.

Gene	Primer sequences	Amplicon size (pb)	Accession number
CCL2	F- TGCTCATAGCAGCCACCTTC R- TCTCCTTGGCCACAATGGTC	189	NM_002982
CCL3	F- CAACCAGTTCTCTGCATCAC R- CTGCTCGTCTCAAAGTAGTC	111	NM_002983
CCL4	F- TCCTCGCAACTTTGTGGTAG R- TCCAGGTCATACACGTACTC	141	NM_002984
CCL20	F- TGATGTCAGTGCTGCTACTC R- GATGTCACAGCCTTCATTGG	146	NM_004591
CXCL2	F- GCCCAAACCGAAGTCATAGC R- AGGAACAGCCACCAATAAGC	154	NM_002089
CXCL10	F- CTAGAACTGTACGCTGTACC R- TTGATGGCCTTCGATTCTGG	175	NM_001565
HPRT	F- ATTGTAATGACCAGTCAACAGGG R- GCATTGTTTTGCCAGTGTCAA	117	NM_000194
IL-1b	F- CTCGCCAGTGAAATGATGGCT R- GTCGGAGATTCGTAGCTGGAT	144	NM_000576
IL-2	F- GTCACAAACAGTGCACCTAC R- TCCTGGTGAGTTTGGGATTC	127	NM_000586
IL-6	F- GTGTGAAAGCAGCAAAGAGG R- TGTTCTGGAGGTACTCTAGG	164	NM_000600
IL-8	F- TTGGCAGCCTTCCTGATTTC R- ATTTCTGTGTTGGCGCAGTG	170	NM_000584
IL-10	F- ACTTTAAGGGTTACCTGGGTTGC R- TCACATGCGCCTTGATGTCTG	111	NM_000572
IL-18	F- GATGGCTGCTGAACCAGTAG R- GCCGATTTCCTTGGTCAATG	191	NM_001562
IL-23p19	F- ATGAGAAGCTGCTAGGATCG R- TTTGAAGCGGAGAAGGAGAC	194	NM_016584
RPL19	F- GGCTCGCCTCTAGTGTCCT R- GCGGGCCAAGGTGTTTTTC	185	NM_000981
TNF-a	F- GCTGCACTTTGGAGTGATCG R- GCTACAACATGGGCTACAGG	132	NM_000594

Primers were designed using Clone Manager software. F: Forward primer; R: Reverse primer. The size of the PCR product is indicated and expected after a two-steps cycle real-time qPCR. Reference genes used for normalization are indicated in bold character. Accession number referred to NCBI website collection.