Figure 1.

IRI dose response curve for standard cell lines.

(A) Cells were exposed to IRI for 72 hours, and viability was determined by SRB assay. The values represent the pooled data from three independent experiments (mean ± SD). The IC50 values and 95% CI given in the main text were calculated from these viability data using a nonlinear regression model in GraphPad Prism.

(B) RNA was isolated from semiconfluent cultures, and the baseline expression of the FGFR3 splice variants IIIb and IIIc was determined by qRT-PCR. The results confirm published data [10].

(C) The correlation between FGFR3-IIIc mRNA levels and IC50 towards IRI was calculated using GraphPad Prism software.