Figure 6.
Combination therapy using IRI and PD173074.
SCID mice were inoculated with 106 HCT116 cells s.c. in their flank. Treatment was initiated when the tumors had become palpable and was done sequentially. Four milligrams per kilogram IRI was injected i.p. twice a week in weeks 1 and 3 (days 13, 16, 27, and 30). Twenty milligrams per kilogram PD173074 was given i.p. four times in week 2 (days 21-24). The experiment was terminated at the end of week 3, and tumors were isolated and snap frozen for the isolation of RNA.
(A) Tumor growth was monitored using a Vernier caliper until day 33 when the animals were sacrificed. It was calculated relative to tumor size on day 13 for each individual tumor, and each data point represents the mean ± SD of three tumors.
(B) RNA was isolated from the snap-frozen tumor tissue, and expression of FGFR3-IIIc, FGF8, and FGF18 was determined by qRT-PCR and calculated relative to the housekeeping gene GAPDH. Bars represent the mean ± SD of three tumors. Differences were analyzed by Student's t test with Welch correction.