Fig. 3.

Comparison of sporulation and fermentation phenotypes of C. pasteurianum-H1 (H1), C. pasteurianum-H1Δspo0A (Δspo0A) and C. pasteurianum-H1-spo0A complementation (spo0A*). a) Spores can be observed in H1 and spo0A* whereas Δspo0A as expected is incapable of producing spores. b), c) Pure glycerol fermentation in serum bottles with 60 g/l glycerol in Biebl medium (b) and CGM (c) was carried out for 48 h with fermentation being visibly completed after 24 h which is the time point shown for glucose consumption and product formation. * indicate statistical significance in t-test α>0.05 of deletion or complementation strain against H1. Error-bars indicate standard error of three replicates.