Fig. 4.

Pure glycerol fermentations of C. pasteurianum-H1 (H1), C. pasteurianum-H1Δrex (Δrex), C. pasteurianum-H1-rex complementation (rex*), C. pasteurianum-H1Δhyd (Δhyd) and C. pasteurianum-H1-hyd complementation (hyd*). a) Bioreactor fermentation with 60 g/l glycerol in Biebl medium with 1 g/l yeast extract at pH 6 was carried out for 48 h with fermentation being visibly completed after 24 h. Error-bars indicate range of two fermentations. b) Histogram showing product formation of serum bottle fermentation of deletion strains and complementations. Glycerol usage and product formation is shown after 24 h. * indicate statistical significance in a t-test α>0.05 of deletion or complementation strain against H1. Error-bars indicate standard error of three replicates.